Amie Holmes, Ph.D., is a full-time assistant research professor at the University of Southern Maine in the Department of Applied Medical Sciences. She received her Ph.D. from the University of Maine in Biochemistry and Molecular Biology, specifically investigating the molecular mechanisms involved in hexavalent chromium-induced carcinogenesis.
Dr. Holmes's work focuses on investigation the molecular mechanisms involved in heavy metal-induced carcinogenesis, specifically focusing on mechanisms of numerical chromosome instability and mitotic disruption. Numerical chromosome instability is a hallmark of many solid tumors and is commonly induced after exposure to environmental carcinogens. Changes in chromosome number can alter the critical balance of proteins required to regulate the cell cycle, chromosome segregation, DNA synthesis and DNA repair. Deregulation of any number of these processes can further destabilize the genome and promote tumorigenesis. Numerous mechanisms exist that induce numerical chromosome instability and most involve disruption of chromosome segregation in mitosis. Her work centers around figuring out how heavy metal exposure disruption normal mitotic progression and induces numerical chromosome instability.
Li Chen T, Lacerte C, Wise SS, Holmes A, Martino J, Wise JP Jr, Thompson WD, Wise JP Sr. Comparative cytotoxicity and genotoxicity of particulate and soluble hexavalent chromium in human and sperm whale (Physeter macrocephalus) skin cells. Comparative Biochemistry and Physiology. Toxicology and Pharmacology, in press.
Holmes AL, Wise JP Sr. Mechanisms of metal-induced centrosome amplification. Biochemical Society Transactions 2010; 38:1678-90.
Wise JP Sr, Wise SS, Holmes AL, Lacerte C, Shaffiey F, Aboueissa AM. The cytotoxicity and genotoxicity of hexavalent chromium in Steller sea lion lung fibroblasts compared to human lung fibroblasts. Comparative Biochemistry and Physiology. Toxicology and Pharmacology 2010; 152:91-8.
Wise SS, Holmes AL, Qin Q, Xie H, Katsifis S, Thompson WD, Wise JP Sr. Comparative genotoxicity and cytotoxicity of four hexavalent chromium compounds in human bronchial cells. Chemical Research in Toxicology 2010; 23:365-72.
Holmes AL, Wise SS, Pelsue SC, Aboueissa A-M, Lingle W, Salisbury J, Gallagher J, Wise JP Sr. Chronic exposure to zinc chromate induces centrosome amplification and spindle assembly checkpoint bypass in human lung fibroblasts. Chemical Research in Toxicology 2010; 23:386-95.